human slc2a2 Search Results


glut2 expression  (OriGene)
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OriGene glut2 expression
(a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of <t>GLUT2</t> in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).
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(a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of GLUT2 in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).

Journal: Nature chemical biology

Article Title: Synthetic beta cells for fusion-mediated dynamic insulin secretion

doi: 10.1038/nchembio.2511

Figure Lengend Snippet: (a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of GLUT2 in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).

Article Snippet: GLUT2 expression and purification The cDNA encoding mouse GLUT2 (Origene, GenBank {"type":"entrez-nucleotide","attrs":{"text":"NM_031197","term_id":"165377236","term_text":"NM_031197"}} NM_031197 ) was amplified and cloned into the BamHI and HindIII sites of pET-28a (Novagen; full plasmid sequence in Supplementary Note ).

Techniques: Concentration Assay, Staining, Labeling, Western Blot